Details-enriched characterization of mobile populations. Morphological details from visuals can increase on the richness of flow cytometry facts. For example, the figure exhibits an usually conventional apoptosis assay applying Annexin V and PI, introducing mobile imaging to characterize cells in Each individual populace to reveal morphologically distinct capabilities. These insights couldn't are obtained from multiplex staining by yourself.
As samples are acquired within the Attune CytPix Flow Cytometer, the superior-speed brightfield camera captures and retailers pictures of detected situations in a charge up to 6,000 pictures for every 2nd, dependant upon the flow level and image dimensions. For bigger overall flexibility, Attune Cytometric Program enables you to regulate the image capture frequency as required and choose a specific gate for impression seize, if essential.
Merge acoustic concentrating technologies with higher-parameter Examination for flexible spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating that has a high-pace brightfield digicam for simultaneous substantial throughput flow cytometry and substantial resolution brightfield imaging.
"An image just isn't very well worth one thousand dots, nevertheless it actually helps you to see pictures. It is excellent to determine pictures that correlate with staining. By way of example, AnnexinV-good cells which might be morphologically diverse than wholesome cells, or CD14+ monocytes which are greater and much more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a highly trained user has gated singlets confidently. After assessing the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate includes a lot more than 4% aggregates.
Pair your Attune flow cytometer using an autosampler for improved workflow performance, and incorporate robotic integration for optimum throughput.
Our researchers will design panels for yourself as often times you would like—totally free, no merchandise acquire needed.
The Attune NxT cytometer is definitely the dependable workhorse instrument your lab can count on for constant and dependable performance. The Attune CytPix design includes a substantial-pace brightfield camera and AI-pushed impression Evaluation software, with recent updates which include machine learning applications and an intuitive consumer interface, enabling Improved info precision, accuracy, and a lot more info quicker insights into sample biology.
Maybe most of all, these gatherings include cells of clearly different phenotypes which could lead on to incorrect conclusions with regards to double good functions (specifically in unusual populations).
AI-pushed automatic image Evaluation Together with the Attune Cytometric Software translates event attributes into distinctive morphology parameters that may be combined with conventional fluorescence and scatter parameters to expose hugely reproducible populace improvements and unique subsets.
We Formerly shown the strength of imaging Auto-T/Ramos cell interactions. Allow’s take a look at just the population of finest fascination, the double positive situations, to learn more. We can easily now use extended morphological parameters (listed here, circularity and skewness of intensity) to more analyze the attributes of those populations and refine gating on these occasions, escalating facts robustness.
Created-in dimensionality reduction instruments increased resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to verify anticipated populace resolution in lysed complete human blood. User can see situations from Every cluster for visual confirmation of cell phenotypes.
No wash/no lyse leukocyte Assessment with imaging. Cells had been obtained from 24-hour-previous blood by dilution in one mM EDTA ( Specialist UX/UI enables people to prepare AI versions for expanded programs. (A) The “Practice Model” aspect presents annotation applications and authentic-time design teaching precision.
Gating on these extended parameters enables you to swiftly and correctly determine populations of interest and make sure gating method with little if any manual assessment. The Attune software package offers entry to point out-of-the-art equipment Finding out Assessment resources inside of an expertly made consumer interface, rendering it extremely easy to use.
The Attune Xenith cytometer is really a high-parameter spectral analyzer, furnishing each spectral unmixing and conventional flow capabilities for detailed and multipurpose cell analysis.
The Attune CytPix Flow Cytometer will allow users to visualise samples through acquisition, confirm the accuracy of gating approaches, and aid outcomes with responsible documentation for publication uses.
What gave the impression to be a homogeneous populace is really much more diverse—an Perception that needs to be viewed as when interpreting effects.